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Annexin V-FITC Apoptosis Detection Kit. Features. ? Annexin V-FITC Apoptosis Detection Kit contains ready-to-use solutions, Annexin V-FITC conjugate, propidium iodide (PI). ? The kit can identify apoptotic and necrotic cells. ? Detect by flow cytometry or fluorescence microscopy. ? No need to fix cells. Mechanism.
For flow cytometry: a 96-well v-bottom plate or FACS tubes for staining and running samples, as appropriate for your flow cytometer; capability of exciting at 488 nm and detecting emission at 525 nm (FITC) and 650 nm. (propidium iodide). 2. For fluorescence microscopy: cell culture plates (or chamber slides) appropriate for
Books Annexin V Fitc Fluorescence Microscopy Kit 550911 Pdf DOWNLOAD NOW annexin v-fitc fluorescence microscopy kit A?A€A” 550911 - bd pharmingenA?A„A? technical data sheet annexin v-fitc fluorescence microscopy kit product information material number: 550911 size: 25 slides component: 51-8074kcab14085
Annexin V-FITC Fluorescence Microscopy Kit. Product Information. Material Number: 550911. Size: 25 slides. Component: 51-8074KC. Annexin V-FITC. Description: Size: 5.0 ml (1 ea). Storage Buffer: Aqueous buffered solution containing BSA and ?0.09% sodium azide. Component: 51-66121E. 10X Annexin V Binding
analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate between apoptosis and necrosis when performing both Annexin V-FITC and PI staining. II. Kit Contents: Components. K78-25. K78-100. K78-400. 25 assays. 100 assays. 400 assays. Annexin V-FITC. 125 µl. 500 µl. 2 ml. 1X Binding Buffer.
prepared by first washing and then incubating with Annexin V-FITC and propidium iodide. The cells are washed again and analyzed by fluorescence microscopy. The combination of Annexin. V-FITC and propidium iodide allows for the differentiation between early apoptotic cells. (Annexin V-FITC positive), late apoptotic
8 Jul 2016 can be easily detected by staining with a fluorescent conjugate of. Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when
tected by staining with fluorescein isothiocyanate (FITC)-labeled annexin V (annexin V-FITC), a protein with a strong, natural affinity for PS. Using this assay, we have detected apoptotic cells in culture, in real time, using fluorescence microscopy and flow cytometry. In combination with vital dye staining, the progressive
Approximate fluorescence excitation/emission maxima: FITC annexin V: 494/518 in nm; Propidium iodide: 535/617 in nm, bound to. DNA. the flow cytometry results by viewing the cells under a fluorescence microscope, using filters appropriate for fluorescein (FITC) and rhodamine (TRITC) or Texas Red® dye. Microscopy.
23 May 2005 See Precautions and Recommendations. Intended Use. The Calbiochem Annexin V-FITC Apoptosis Detection Kit is a non-isotopic system for the identification of cell membrane alterations that accompany programmed cell death. Detection may be performed by flow cytometry or by fluorescence microscopy
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